Leaf tissue of Glycine max Merr. was fixed in para-formaldehyde-glutarldehyde and postfixed in osmium tetroxide or postassium permanganate for electron microscopy.
The origin of cytolysome-like organelles of mesophyll cell was studied and changes of fine structure of the organelles according to treating solutions such as gibberellin(GA), kinetin(KI), 2,4-dichlorophenoxy acetic acid(2,4-D) or 2,4-D+GA(1㎎/ℓ, respectively) were observed.
The cytolysome-like organelles differentiate in endoplasmic reticulum and plasmalemma, and they drop into vacuoles being isolated from the formers. They seem to change into myelin-like structure and to be degenerated by autodigestion.
Cytolysome-like organelles involved in cell walls and vacuoles showed activity of acid phosphatase.
In the group of GA and KI treatment, cytolysome-like organelles were similar to that of the control group. But in the treatmental groups of 2,4-D and 2,4-D+GA, myelin-like structures increased in size and autodigestion of this organelles were similar to that of the control group. But in the treatmental groups of 2,4-D and 2,4-D+GA, myelin-like structures increased in size and autodigestion of this organelle seemed to be accelerated.
In the treatmental group of 2,4-D+GA, myelin-like structures shown high electron density were observed in cytoplasm and vacuoles together.
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